COMPARATIVE ANALYSIS OF THE ANTIMICROBIAL EFFICACY OF AYURVEDIC SEEDS IN WASTEWATER FILTRATION

Authors

  • Tanish Veernapalli High School Students, Biochemistry, Indus International School Hyderabad, Shankarpalle, India
  • Saketh Kollipara High School Students, Biochemistry, Indus International School Hyderabad, Shankarpalle, India
  • Tara Pratapa High School Students, Biochemistry, Indus International School Hyderabad, Shankarpalle, India

DOI:

https://doi.org/10.21276/IERJ24697287410876

Keywords:

Strynchnos Potatorum, Moringa Oleifera, Slavia Hispanica, Foeniculum Vulagare Mill, Sustainable Water Purification, Antimicrobial Properties

Abstract

Waterborne diseases such as Hepatovirus, Vibrio Cholera, Shigella, Salmonella, Campylobacter, and Escherichia coli pose significant public and individual health challenges in underdeveloped areas lacking access to safe and clean drinking water, as mentioned in the UN Sutainable Develepment Goal Number 6, which highlights the need for sustainable and cheap water purification methods. This experiment tested the antimicrobial properties of the following ayurvedic  (i) Strychnos Potatorum, (ii)Moringa Oleifera,(iii) Salvia hispanica,(IV) Foeniculum vulgare Mill, by taking stable concentrations of each seed extract and testing them against dirty water samples collected from polluted water sources. Water samples have been mixed with definite concentrations of extract, spread under agar, and then incubated for 24 hours to identify bacterial inhibition zones. After 24 hours of incubation, a Manual colony counter LMCC-A11 was used to identify major countable colonies. The sample of Strynchnos Potatorum showed the greatest bacterial inhibition, followed by Foeniculum Vulgare Mill, Slavia Hispanica, and Moringa Oleifera. Compared to dirty water, the seeds inhibited what was nearly 500-700 colonies of bacteria that could have been present in the samples.With the results above, seeds, ayurveda sample Strynchnos Potatorum, showed the most antimicrobial activity against the various bacteria and harmful compositions present in dirty water, representing its strong potential in being able to filter microbes in water to greater extents. This investigation addresses the importance of sustainable and cheaper methods of water filtration by diving into the antimicrobial aspects of various plant extracts against contaminated water, highlighting its replaceability with traditional filtration methods

References

I. “What Are the Main Sources of Water Pollution?” European Environment Agency, 2024,

II. Peng, Xiaoxuan & Jiang, Yushan & Chen, Zhonghao & Osman, Ahmed & Farghali, Mohamed & Rooney, David & Yap, Pow Seng. (2023). Recycling municipal, agricultural and industrial waste into energy, fertilizers, food and construction materials, and economic feasibility: a review. Environmental Chemistry Letters. 21. 1-37. 10.1007/s10311-022-01551-5.

III. Vijay, Kalekar Sumita and Shinde Nanda Waman. “Use Of Strychnos potatorum Seed Powder As A Water Purifier: A Sustainable Approach For Rural Development.” Global journal for research analysis 4 (2015): n. pag.

IV. Yadav KN, Kadam PV, Patel JA, Patil MJ. Strychnos potatorum: Phytochemical and pharmacological review. Pharmacogn Rev. 2014 Jan;8(15):61-6. doi: 10.4103/0973-7847.125533. PMID: 24600197; PMCID: PMC3931202.

V. S. Mandal, A. Kunhikrishnan, N.S. Bolan , H. Wijesekara , R. Naidu. Application of Biochar Produced From Biowaste Materials for Environmental Protection and Sustainable Agriculture Production, 10.1016/B978-0-12-803837-6.00004-4

VI. MALLIKHARJUNA, P. & Seetharam, YN. (2009). In Vitro Antimicrobial Screening of Alkaloid Fractions from Strychnos potatorum. Journal of Chemistry. 6. 10.1155/2009/535643.

VII. Yan Y, Li X, Zhang C, Lv L, Gao B, Li M. Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review. Antibiotics (Basel). 2021 Mar 19;10(3):318. doi: 10.3390/antibiotics10030318. PMID: 33808601; PMCID: PMC8003525.

VIII. Anzano A, de Falco B, Ammar M, Ricciardelli A, Grauso L, Sabbah M, Capparelli R, Lanzotti V. Chemical Analysis and Antimicrobial Activity of Moringa oleifera Lam. Leaves and Seeds. Molecules. 2022 Dec 15;27(24):8920. doi: 10.3390/molecules27248920. PMID: 36558052; PMCID: PMC9782826.

IX. Dilika, F et al. “Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites.” Fitoterapia vol. 71,4 (2000): 450-2. doi:10.1016/s0367-326x(00)00150-7

X. Hafiz Abubaker Saddiqi, Zafar Iqbal. Chapter 55 - Usage and Significance of Fennel (Foeniculum vulgare Mill.) Seeds in Eastern Medicine. DOI: 10.1016/B978-0-12-375688-6.10055-6

XI. Tamokou, Jean De Dieu & Mbaveng, Armelle & Kuete, Victor. (2017). Antimicrobial Activities of African Medicinal Spices and Vegetables. 10.1016/B978-0-12-809286-6.00008-X.

XII. Badgujar SB, Patel VV, Bandivdekar AH. Foeniculum vulgare Mill: a review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology. Biomed Res Int. 2014;2014:842674. doi: 10.1155/2014/842674. Epub 2014 Aug 3. PMID: 25162032; PMCID: PMC4137549.

Additional Files

Published

15-06-2024

How to Cite

Tanish Veernapalli, Saketh Kollipara, & Tara Pratapa. (2024). COMPARATIVE ANALYSIS OF THE ANTIMICROBIAL EFFICACY OF AYURVEDIC SEEDS IN WASTEWATER FILTRATION. International Education and Research Journal (IERJ), 10(6). https://doi.org/10.21276/IERJ24697287410876