• Madhuri Singhal Department of Chemistry, Govt. S.G.S. Post Graduate College, Ganjbasoda India
  • Jagadamba Singh Department of Chemistry, Allahabad University, Allahabad India
  • Anjali Jijhotiya Department of Chemistry, Govt. S.G.S. Post Graduate College, Ganjbasoda India


13CNMR, four days uteri weight assay, EIMS, estrogenic activity, extraction, 1HNMR, isolation, isoflavone, Lawsonia inermis Linn


An isoflavonoid has been isolated from the stem extract of Lawsonia inermis Linn. (Lythraceae). The isoflavonoid tested on female albino rats, showed estrogenic activity by increasing uteri weight from 16.5 ± 0.28 mg in control to 34.4 ± 0.69 mg in orally treated rats. Air-dried and powdered stem of Lawsonia inermis was extracted with hot rectified spirit. The extract obtained was evaporated to dryness. The residue obtained was subjected to successive extraction with petrolium ether, benzene, chloroform, ethyl acetate, and methanol. The residue of the methanol extract was subjected to column chromatography over silica gel column with chloroformmethanol mixtures. The 7:3 chloroform-methanol elute was distilled in order to remove the solvent affording pale yellow crystals of compound. The compound has been identified as the isoflavonoid genistein by the joint application of chemical methods and spectroscopic methods (UV, IR, 1HNMR, and 13CNMR). The compound was obtained as a pale yellow mass, which was re-crystallised by methanol, analysed for C15H10O5 m.p, 285-287.The crude extract and methanol solution of the compound on screening by “four day uterine weight assay” showed positive estrogenic activity. Result showed the dose dependent estrogenic activity of compound by increasing uterine weight from 15.6 ± 0.26 mg in orally treated female albino rats (1.25 mg/kg/day for four days).


Cheng XM (1993). Annual Reports of Medicinal Chemistry. Washington DC, pp. 331-

Chopara RN, Nayar SL, Chopra IC (2002). Glossary of Indian Medicinal Plants. New

Delhi, CSIR Publication, pp. 151.

Kaufman PB, Duke JA, Brielmann H, Boik J, Hoyt JE (1997): A comparative survey of

Leguminous plants as sources of the isoflavones, genistein and daidzein: Implications for

human nutrition and health. The Journal of Alternative and Complementary Medicine.

(1): 7-12.

Markham KR (1982). Techniques of flavonoid identification. New York, Academic

press, pp.15-16

Saxena VK, Singhal M (1998). Geinstein 4'-O-alpha-L rhamnopyranoside from

Pithecellobium dulce. Fitoterapia 69(4): 305-306.

Turner A, Hebborn P (1971). Screening Methods in Pharmacology. New York, II

Academic Press, pp. 93.

Thompson EB (1990): Drug Bioscreening: Drug evaluation techniques in Pharmacology.

New York, VCH Publisher, pp. 299-303.

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How to Cite

Madhuri Singhal, Jagadamba Singh, & Anjali Jijhotiya. (2016). ESTROGENIC ACTIVITY OF ISOFLAVONOID OBTAINED FROM LAWSONIA INERMIS. International Education and Research Journal (IERJ), 2(6). Retrieved from