ESTROGENIC ACTIVITY OF ISOFLAVONOID OBTAINED FROM LAWSONIA INERMIS

Authors

  • Madhuri Singhal Department of Chemistry, Govt. S.G.S. Post Graduate College, Ganjbasoda India
  • Jagadamba Singh Department of Chemistry, Allahabad University, Allahabad India
  • Anjali Jijhotiya Department of Chemistry, Govt. S.G.S. Post Graduate College, Ganjbasoda India

Keywords:

13CNMR, four days uteri weight assay, EIMS, estrogenic activity, extraction, 1HNMR, isolation, isoflavone, Lawsonia inermis Linn

Abstract

An isoflavonoid has been isolated from the stem extract of Lawsonia inermis Linn. (Lythraceae). The isoflavonoid tested on female albino rats, showed estrogenic activity by increasing uteri weight from 16.5 ± 0.28 mg in control to 34.4 ± 0.69 mg in orally treated rats. Air-dried and powdered stem of Lawsonia inermis was extracted with hot rectified spirit. The extract obtained was evaporated to dryness. The residue obtained was subjected to successive extraction with petrolium ether, benzene, chloroform, ethyl acetate, and methanol. The residue of the methanol extract was subjected to column chromatography over silica gel column with chloroformmethanol mixtures. The 7:3 chloroform-methanol elute was distilled in order to remove the solvent affording pale yellow crystals of compound. The compound has been identified as the isoflavonoid genistein by the joint application of chemical methods and spectroscopic methods (UV, IR, 1HNMR, and 13CNMR). The compound was obtained as a pale yellow mass, which was re-crystallised by methanol, analysed for C15H10O5 m.p, 285-287.The crude extract and methanol solution of the compound on screening by “four day uterine weight assay” showed positive estrogenic activity. Result showed the dose dependent estrogenic activity of compound by increasing uterine weight from 15.6 ± 0.26 mg in orally treated female albino rats (1.25 mg/kg/day for four days).

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Additional Files

Published

15-06-2016

How to Cite

Madhuri Singhal, Jagadamba Singh, & Anjali Jijhotiya. (2016). ESTROGENIC ACTIVITY OF ISOFLAVONOID OBTAINED FROM LAWSONIA INERMIS. International Education and Research Journal (IERJ), 2(6). Retrieved from https://ierj.in/journal/index.php/ierj/article/view/294