• Akintola A. O Department of Science Laboratory Technology,LadokeAkintola University of Technology, Ogbomoso, Nigeria.
  • Maduagwu E.N Department of Biochemistry, Faculty of Basic Medical Sciences University of Ibadan Nigeria
  • Adegoke A.O Department of Pharmaceutical Chemistry, University of Ibadan, Ibadan, Nigeria.
  • Kehinde B. D Department of Biochemistry, Faculty of Basic Medical Sciences, LadokeAkintola University of Technology, Ogbomoso, Nigeria.
  • Ademowo O.G Institutefor Advanced Medical Research and Training, College of Medicine, University of Ibadan, IbadanNigeria.


Crinum jagus is a medicinal plant used traditionally in Nigeria to treat infectious diseases such astuberculosis andmalaria. In the present study, the antimicrobial properties of the crude extract and chromatographic fractions from the bulb of Crinum jaguswere investigated against clinical and laboratoryisolates of bacteria and fungi using both agar well diffusion and agar dilution methods.Ampicillin (antibacterial) and tiaconazole(antifungal) were used as positive reference standard drugs.The crude plant extract and its fractionsdemonstrated broad spectrum activityagainst all the bacteriaand fungi isolatestested. Fraction 1 (24.00 mm zone of inhibition, MIC: 0.20 µg/mL, MBC:0.39µg/mL, MFC: 0.78µg/mL)demonstrated the highest activity, followed by Fraction 2 (24.00mm zone of inhibition, MIC: 0.39µg/mL, MBC: 0.78µg/mL, MFC: 1.56µg/mL. Fraction 3 (20:00mm zone of inhibition, MIC: 0.78µg/mL, MBC: 0.78µg/mL, MFC: 1.56µg/mL,). The crude extract howeverdemonstratedthe least activity against the test bacteria and fungi (18.00 mm zone of inhibition,MIC: 6.25mg/mL, MB: 25.00mg/mL, MFC: 50mg/mL.Preliminary phytochemical analysis revealed the presence of alkaloids, phenols, flavonoids,saponinsand steroids which may account for the antimicrobialactivity of theplant. The result of the study demonstrated that the extractand fractions of the bulb of Crinum jagus has appreciable antimicrobial properties and suggest that it may be useful in the treatment of microbial infections.


Adesanya, S.A., Olugbade, T.A., OdebiyiO.O. and Aladesanmi J.A. (1992): Antibacterial alkaloids in Crinum jagus. International Journal of Pharmacology ; 4 : 303-307.

Adesokan, A.A., Akanji, M.A., Yakubu, M.T. (2007): Antibacterial potentials of aqueous extract of Enantiachiorantha stem bark. Africa Journal of Biotechnology ; 6 (22): 2502 – 2505.

Alonso, R., Fernandez Arangalz, A., Colom K., Herreras, A. and Gsterna R. (2000) : Profile of bacterial collates and antimicrobial susceptibility. Multi-centerstudy using one day of cut-off.RevistaEspanoladeQuimioterapia; 13: 384-393.

Alviano D.S. and Alviano C.S. (2009) : Plant extracts; search fornew alternatives to treat microbial diseases.Current Pharmaceutical Biotechnology : 106-121.

Azikwe, C.C.A.,Siminilayi, M., Brambaifa, N., Amazu L.U., Enye, J.C. and EzeaniM.C. (2012). Anticonvulsant activity of fractionated extract of Crinum jagus bulbsin experimental animal. Asian Pacific Journal of Tropical Biomedicine ; 9: 21-29.

Black, J.G. (1996): Microbiology: Principles and Application.Prentice Hall, New York. p.260.

Budlack,W.R., Omaye, S.T., Meskin, M.S, and Topham. D.K.W. (2000) : Phytochemicals as bioactive agents.CRC press, Boca Raton,. FL

Cowan, M.M (1999) : Plant products as antimicrobial agents . Clinical Microbial Review ; 12 : 564-582.

Edoeoga H.O., OkwuD.E. and Mbaebie B.O. (2005) : Phytochemical constituent of some Nigeria medicinal plants. African Journal of Biotechnology; 4 (7) : 685 -688.

Gangoue – Pieboji, J., Pegnyemb, D.E., Niyiteka, D. (2006): The in-vitro antimicrobial; activities of some medicinal plants from Cameroon.Annual Tropical Medicinal Parasitology ; 100: 237-243.

GradamM.A. (2000): Is methicillin resistant Staphylococcus aureus an emerging community pathogen?. A review of the literature.The Canadian Journal of infectious Diseases ; 11: 202 -211.

Gupta, P., Murali, P, Murali, M.V.,Faride, N.M., A., Kaul, P.B., Ramachandram V., C., TalwarV. (1993): Clinical profile of Klebsiellasepticerniain neonates.Indian Journal of Paediatrics; 60: 565-572.

Hassan, M.M., Oyewale, A.O., AmupitanJ.O., Abdullahi, M.S., Okonkwo, E. M. (2004):Preliminary phytochemical and antibacterial investigation of crude extracts ofthe root bark of Devatiummicrocarpum. Journal of Chemical Society of Nigeria ; 29: 26-29.

Hermaiswaya, S., Kruthiventi, and Dobie M.C. (2008) : Synergism between natural products and antibiotic against infectious diseases.Phytomedicine;15: 639 -652.

Idu, D., ErhaborJ.O.,Efijueme H.M. (2010) : Documentation of medicinal plants sold in markets in Abeokuta, Nigeria. Tropical Journal of Pharmaceutical Research : 9 (2) : 110 -118.

Lino, A. and Deogracious O. (2006): The in-vitroantibacterial activity of Annona senegalensissecuridaccalongipendiculataand Steanotaeniaaralliacea-UgandanMedicinal plants.African Health Science ; 6 (1): 31-35.

Mabberly, D.(1990) : Theplant book Cambridge University Press.

Maliady G.B.(2005) : Medicinal plants for the prevention and treatment of bacteria infection. Current Pharmaceutical Design ; 11: 2405-2427.

Mastroeni, P (2002 : Immunity to systemic Salmonella infections. Current Molecular Medicine ; 2: 393 -406.

Monroe, S. and Polk, R. (2000) : Antimicrobial use of bacterial resistance. Current opinion in Microbiology; 3:496-501

Ode, O.J and Asuzu, O. (2006) : The anti-snake venom activity of the methanolicextract of thebulb of Crinum jagus. Toxicon; 48: 331-342.

Ode, O.J., Nwaehuger, C.O. and Onakpa. M.M. (2010) : Evaluation of antihaemorrhagic and antioxidant potentials of Crinum jagusbulb. International Journal of Applied Biology and PharmarceuticalTechnology ; 1 (3) : 1330- 1336.

Ogbole, J.N., Ogeke, C.C., Okoli, I.C., Anyawu B.N (2007): Antibacterial activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta. African Journal of Biotechnology; 6 (13): 1544 -1548

Owolabi, O.J., Omogbai, E.K.I., ObasuyiO. (2007): Antifungal and antibacterial activities of the ethanolic and aqueous extracts of Kigellaafricana(Bignoniace) stembark. African Journal of Biotechnology; 6 (14) : 1677-1680.

Oyeleke, S.B., Dauda, B.E.N. and Boye O.A (2008): Antibacterial activity of Ficuscapensis. African Journal of Biotechnology ; 7 (10) : 1414-14-17.

Peter, J.H., Joseph, M.A. and Adegbulugbe, A. (2004): Choline esterase inhibitory properties of alkaloids from two Nigerian Crinum species. Phytochemistry; 65 : 2893-2896.

Sader, H.S., Jones, R.N and Silva, J.B. (2002) : Skin and soft tissues infections in Latin America medicinal centre.Four year assessment of the pathogen frequency and antimicrobial susceptibility.Diseases; 44; 281 – 288.

Sahm, D.F and Washinton J.A. (1990) : Antibacterial susceptibilitytest; Dilution methods in manual of clinical Microbiology Lennette E.H(ed.)5thedition. American Society of Microbiology. Washington DC pp 1105 -1118.

Shiba, H., Kondo, K., Katsuyama, R. (2005) : Alkyl Gallates, Intesifer of β-lactam susceptibility in methicillin-resistant Staphylococcus aureus.Antimicrobial Agents Chemotherapy; 49 : 549-555.

Usman,H, and Osuji, J.C. (2007): Phytochemical an in–vitroantimicrobialassay of the leaf extract of Newbouldialeaves. African Journal of Traditional and Complementary Alternative Medicine;4 (4): 467-480.

WHO (2013): Mortality and global health estimates. Geneva Swizerland: World Health Organisation.

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How to Cite

Akintola A. O, Maduagwu E.N, Adegoke A.O, Kehinde B. D, & Ademowo O.G. (2016). ANTIMICROBIAL ACTIVITIES OF CRUDE METHANOLIC EXTRACT AND FRACTIONS OF THE BULB OF CRINUM JAGUS (LINN). International Education and Research Journal (IERJ), 2(6). Retrieved from