• Sangale PP Department of Botany, Post Graduate Research Center Chandmal Tarachand Bora College, Shirur (Ghodnadi), Shirur, Dist. Pune
  • Devikar SD Department of Botany, Post Graduate Research Center Prof. R. M. College Akurdi, Pune
  • Naikawadi VB Department of Botany, Post Graduate Research Center Chandmal Tarachand Bora College, Shirur (Ghodnadi), Shirur, Dist. Pune
  • Ghawate AN Department of Botany, Post Graduate Research Center Prof. R. M. College Akurdi, Pune


Clonal propagation, axillary shoot, differentiation, eco-restoration immune-buster tonic herb, anti-inflammation


Sphaeranthus gomphrenoides (Gorakhmundi; Asteraceae) is a unique medicinal plant species, which found in fragile ecosystem near river side. It has unique importance in Indian cultural festivals as well in ethno-medicinal practices. In Ayurveda, whole plant has shown tremendous medicinal properties and used as immune-buster tonic, eyes diseases, urine disease, skin diseases, pulmonary diseases and inflammation diseases.  Oil extraction from the roots and inflorescence has been used in scrofula and generally useful as aphrodisiac by the local community. Application plant part paste is beneficial on arthritis and edema. As it is commonly used by the local people and it is found only in rainy season aside of riverine seasonal wet land area, so this species to be hampered. Under the in vitro condition, plant growth regulators with MS medium play an important for morphogenesis in various medicinal plant species. Moderate concentration of BAP with MS medium was on node explants showed better shoot regeneration percentage with healthy shoots formation.  Application of high concentration of BAP was enhanced percentage of callus induction and declines the shoot regeneration capacity of nodal explants. Second sub-cultured nodal callus showed small shoot buds on low concentration of BAP with MS + 35 % sucrose.  Compare with callus mediated shoots with direct shoots from node show higher percentage of healthy shoots formation. This report is on influence of BAP for mass multiplication of S. gomphrenoides. The developed protocol may be useful for improvement and eco-restoration of S. gomphrenoides in that particular niche for sustainable utilization.


I. Kadapatti, S., Murthy, H. (2023) Micropropagation of threatened medicinal plant Andrographis lineata var. lawii. Vegetos.

II. Martin, K. (2002) Rapid propagation of Holostema ada‐kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Rep. 21:112‐117.

III. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–505

IV. Naikawadi, V. B., Ahire, M. L., Lahiri, A., Nikam, T. D., (2016). In vitro propagation and cell cultures of memory tonic herb Evolvulus alsinoides: a best source for elicited production of scopoletin. Applied microbiology and biotechnology, 100, 3463-3476.

V. Nikam, T. D., Ghorpade, R. P., Nitnaware, K. M., Ahire, M. L., Lokhande, V. H.,Chopra, A., (2013). Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb. Physiology and Molecular Biology of Plants, 19, 105-116.

VI. Nithya, V., Kamalam, M. (2021) Standardization of a protocol for micropropagation of Eupatorium glandulosum L. an important medicinal plant. Plant Cell Tiss Organ Cult 146, 339–344.

VII. Norrizah, S., Hidayah, W., Aminah, S., Ruzaina, S., & Faezah, P. (2012). Effect of medium strenght and hormones concentration on regeneration of Pogostemon cablin using nodes explants. Asian J. of Biotech, 4(1), 46-52.

VIII. Patil, J., Ahire, M., Nitnavare, K., Panda, S., Bhatt, V., Kavi, P., Nikam, T. (2013) In vitro propagation and production of cardiotonic glucosides in shoot cultures of Digitalis purpurea L. by elicitation and precursor feeding. Appl Micobiol Biotechnol; 97: 2379-2393.

IX. Shekhawat, MS., Kannan, N., Manokari, M., Ravindran, C. (2015) In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures. J Genet Eng Biotechnol 13(2):209–214.

X. Wesely., E, Johnson., M, Regha P and Kavitha M. (2010) In vitro propagation of Boerhaavia diffusa L. through direct and indirect organogenesis J. Chem. Pharm. Res., 2(5):339-347.

XI. Yu, L., Duan, Y. & Zhan, H. (2023) An improved micropropagation of a medicinal plant Stephania dentifolia. Plant Cell Tiss Organ Cult 153, 219–224.

XII. Sardesai, M., Girdhari, M., Nimbalkar, V., Sharma, O. (2021) Sphaeranthus gomphrenoides (Compositae): New record to Asia with its identity, typification, and distribution, phytotaxa.514.3.9/ Vol. 514 No. 3: 17.

XIII. Mahajan, N., Chopda, M., Mahajan R. (2015) A Review on Sphaeranthus Indicus Linn: Multipotential Medicinal Plant, International Journal of Pharmaceutical Research & Allied Sciences, Volume 4, Issue 3 (2015):48-74, ISSN 2277-3657

XIV. Ramachandran, S. (2013) Review on Sphaeranthus indicus Linn, pharmacognocy reviews,pubmed,Vol-7,Issue-14-2013,ISSN -0973-7847.

XV. Sultana A, Fazmiya M , Rahman K (2021) Mundi (Sphaeranthus indicus Linn): The best blood purifier and immunomodulatory Unani herb with versatile ethnomedicinal uses and pharmacological activities, cellmed, 2021 / Volume 11 / Issue 2 / 10.

Additional Files



How to Cite

Sangale PP, Devikar SD, Naikawadi VB, & Ghawate AN. (2023). IN VITRO REGENERATION OF SPHAERANTHUS GOMPHRENOIDES: A ETHNOMEDICINAL HERB SPECIES. International Education and Research Journal (IERJ), 9(5). Retrieved from