• K.Punitha Ph.D., Research Scholar (Full time), Reg.No:21212102102012, PG and Research Department of Microbiology, Kamaraj College, Thoothukudi. (Affiliated to Manonmaniam Sundarnar University, Tirunelveli), Tamil Nadu, India.
  • M.Sangeetha Assistant Professor, PG and Research Department of Microbiology, Kamaraj College, Thoothukudi. (Affiliated to Manonmaniam Sundarnar University, Tirunelveli), Tamil Nadu, India.


Chitosan, marine fish, wound healing, FTIR


Fish scales are one of the most underrated material, which is compiled with numerous benefits. India is the leading countries in marine fishing, by utilizing the marine fish scale wastes as the source of chitosan. It is an active biopolymer withholds promising benefits. Wound healing is one of the significant process, given much importance because of the microbe invasion makes the process complicated. The extracted chitosan shows efficient yield percentage of about 58.42%. the highest inhibitory zone is 15mm against S.aureus. chitosan is characterized through FTIR analysis, and formulated into a gel to promote wound healing activity.


I. Ait M, Chairi H, Laglaoui A, Arakrak A, Zantar A, Bakkali M, Hassani M (2018) Optimization and characterization of gelatin and chitosan extracted from fish and shrimp waste. E3S Web of Conference 37, 02006.

II. Boarin-Alcalde L, Fonseca GG (2016) Alkali process for chitin extraction and chitosan production from Nile tilapia (Oreochromis niloticus) scales. Lat Am J Aquat Res 44:683-688.https://doi:10.3856/vol44-issue4-fulltext-3

III. Gokulalakshmi E, Ramalingam K, Umasankari, Vanitha MC (2017) Extraction and characterization of chitosan obtained from scales of Clarias gariepinus (catfish). Biotechnol J Int 18:1-8.

IV. Prameela K, Murali mohan CH, Smitha PV, HemalathaKPJ (2010). Bioremediation of shrimp biowaste by using natural probiotic for chitin and carotenoid production an alternative method to hazardous chemical method. IJABPT. 2010;3:0976-4550

V. Kumari S, Annamareddy S.H.K, Abanti S, Rath PK (2017) Physiochemical properties and characterization of chitosan synthesized from fish scales, crab and shrimp shells. Int J Biol Macromol 104:1697-1705. https://doi:10.1016/j.ijbiomac.2017.04.119

VI. Anand S, Snigdha S, Sukanya D, Deepshikha D (2018) Extraction and characterization of chitosan from waste scales of Labeo Rohita. JETIRC006096 (ISSN- 2349-5162)

VII. Jong-Whan Rhim, Seok-In hong, Hwan-Man Park Perry K.W. Ng (2006) Preparation and Characterization of chitosan-based nanocomposite films with antimicrobial activity.

VIII. Younes I, Rinaudo M (2015). Chitin and chitosan preparation from marine sources, Structure properties and applications. Marine drugs 2015;13.

IX. Younes I, Sellimi S, Rinaudo M, Jellouli K, Nasri M (2014). Influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities. Internaltional journal of food microbial 2014;185.

X. Dai T, Tanaka M, Huang Y.Y, Hamblin M.R (2011). Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects. Expert review of anti-infective therapy.

XI. Jayakumar R, Reis R.L, Mano J.F (2006). Phosphorous containing chitosan beads for controlled oral drug delivery. Journal of bioactive and compatible polymers.

XII. Annaian S, Kandasamy K, Lakshman N (2016). Preparation, characterization and antibacterial activity of chitosan and phosphorylated chitosan from cuttlebone of Sepia kobiensis (Hoyle, 1885). Biotechnology reports.

XIII. Aichayawanich S, Saengprapaitip M (2019). Isolation and characterization of chitosan from fish scale waste. IOP conference series Earth and environmental science. https://doi:10.1088/1755-1315/301/1/012051

XIV. Takarina N.D, Fanani A.A (2016). Characaterization of chitin and chitosan synthesized from red snapper(lutjanus sp.) scale’s waste. IOP conference proceedings.

XV. Abdou, E.S.; Nagy, K.S.A.; Elsabee, M.Z (2008) Extraction and characterization of Chitin and Chitosan from local sources. Bioresour. Technol.

XVI. No H.K, Cho Y.I, Kim H.R, Meyers S.P. Effective deacetylation of chitin under 72 conditions of 15 psi/1210C. J agric Food Chem.2000;48:2625-2627.

XVII. Gaur R, Azizi M, Gan J, Peter H (2009). Simple ointment: formulated preparations.

XVIII. Vigneshwari S, Gokula V. Extraction and FTIR characterization of chitosan from Portunus pelagicus (Linnaeus, 1758) shell wastes S. Int j Adv. 2018;3(10):91-95

XIX. Peers S, Montembault A, Ladaviere C. Chitosan hydrogels for sustained drug delivery. J Control Release. 2020;326:150-163.

XX. Kumar M.N.V Ravi (2000) A review of chitin and chitosan applications. Reactive & Functional Polymers vol 46pp 1-27.

XXI. Peipei F, Yang L, Chunhai K, Haofeng Q,Wei W, Yabin Z, Ruixia H,Long X, Sondze W (2021). Chitosan- based functional materials for skin wound repair:mechanisms and applications. Biomaterials. Hhtps://

Additional Files



How to Cite

K.Punitha, & M.Sangeetha. (2023). THE UTILIZATION & CHARACTERIZATION OF MARINE FISH SCALE CHITOSAN AS A GEL FOR WOUND HEALING ACTIVITY. International Education and Research Journal (IERJ), 9(5). Retrieved from