STUDY OF ACTN-3 GENE IN POWER ORIENTED ATHLETES
Keywords:α-actinin-3 (ACTN3) R577X Gene, Power Athletes, Gradient PCR, Athletic Performance, DNA Bank, Molecular analysis
Physique, diet and training regime have been long-established parameters influencing athletic performance, whereas, the genetic configuration is another crucial factor that can give insight into the physical capabilities of an individual at an earlier stage of life. Several genes have been linked to different aspects of physical performance. One prominent association is seen with a gene called α-actinin-3 (ACTN3) R577X that is normally turned on in a type of muscle fiber (fast-twitch) used for power-based sports.
There are three possible genotypes of ACTN3 - RR, RX and XX. It has been hypothesized that RR and RX is associated with power-based performance. To analyze this theory, we studied the ACTN3 gene distribution in total 110 young individuals from Maharashtra region involved in power-based sports orientation and sedentary individuals as control. Genomic DNA bank was prepared from their blood samples followed by PCR amplification of ACTN 3 gene. It is evident from previous reports that RR and RX alleles of ACTN3 influence power-oriented athletics, which can be established by genotypic investigations such as RFLP and considering larger sample population. Early genetic inquiries may assist in channelizing the sporting abilities and management of injuries of budding athletes, when used with more generic factors like nutrition, training regimes and coaching.
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