Hajirabanu R, Dr. Nausheen Dawood, Dr. Sultan Ahmed Ismail


DNA barcoding is a powerful tool used for species identification by the expanding exploitation of mtDNA gene cytochrome c oxidase I (COI), as a genetic marker. In the present study, COI gene based DNA barcoding was employed to identify the epigeic species Eudrilus eugeniae. Molecular characterization of Eudrilus eugeniae was performed using Polymerase Chain Reaction (PCR) for COI gene. Phylogenetic tree was constructed using Molecular Evolutionary Genetics Analysis (MEGA7) software for the obtained sequences. The evolutionary divergences between its closely related species were also performed to disclose its amendments that occurred during evolution.

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Hebert PDN, Cywinska A, Ball SL, DeWaard JR. Biological identifications through DNA barcodes. Proc R Soc Lond B 2003;270:313‐321.

Hajibabaei M, Singer GAC, Clare EL, Hebert PDN. Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. BMC Biol 2007;5:1‐24.

Symondson WOC. Molecular identification of prey in predator diets. Mol Ecol 2002;11:627–641.

Banu JR, Logakanthi S, Vijayalakshmi GS. Biomanagement of paper mill sludge using an indegenous(Lampito mauritii) and two exotic (Eudrilus eugineae and Eisenia foetida) earthworms. J Environ Biol 2001;22:181-185.

Gajalakshmi S, Ramasamy EV, Abbasi SA. Potential of two epigenic and two anecic earthworm species in vermicomposting of water hyacinth. Bioresour Technol 2001;76:177-181.

Tsai CF, Tsai SC, Liaw GJ. Two new species of protandric pheretimoid earthworms belonging to the genus Metaphire (Megascolecidae: Oligochaeta) from Taiwan. J Nat Hist 2000;34:1731–1741.

Nagavelamma KP, Wani SP, Stephance L, Padmja W, Vineetha C, Babu Rao M, et al. Vermicomposting: Recycling wastes into valuable organic fertilizer. International Crops Research Institute for the Semi-Arid Tropics 2004;2:20.

Navot N, Zeidan M, Pichersky E, Zamir D, Czosnek H. Tomato yellow leaf curl virus: A whitefly-transmitted geminivirus with a single genomic component. Virology 1991;185:151-161.

Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwach W. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. PNAS 2004;101:41.

Rubinoff D, Cameron S, Will K. Are plant DNA barcodes a search for the Holy Grail? Trends Ecol Evol 2006;21:1.

Lunt DH, Zhang DX, Szymura JM, Hewitt GM. The insect cytochrome oxidase I gene:evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 2006;5:153-165.

Sass C, Little DP, Stevenson DW, Specht CD. DNA Barcoding in the Cycadales: Testing the Potential of Proposed Barcoding Markers for Species Identification of Cycads. PLoS ONE 2007;11:1154.

Amendt J, Krettek R, Zehner R. Forensic entomology. Naturwissenschaften 2004;91:51-65.

Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10:512-526.

Felsenstein J. Confidence limits on phylogenies:An approach using the bootstrap. Evol 1985;39:783-791.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0. Mol Biol Evol 2016;33:1870-1874.


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