TOXICITY OF METALS SUCH AS IRON, NICKEL AND COBALT ON SEEDLINGS OF BROAD BEANS (VICIA FABA L.)
Keywords:Ni, Fe, Co, toxicity, phosphatase, amylase
Increased industrialization in developing countries like India has resulted into the enormous deterioration of air, water and soil. Presence of heavy metals causes bioaccumulation affecting the entire ecosystem and pose harmful consequences. On treating beans with increasing concentration (10mM, 50mM, 100mM, 150mM) of heavy metals (Ni, Co,Fe) significant decrease (p≤0.05) in seed germination, root length, shoot length, root and shoot length and dry weight was observed but there was significant increase (p≤0.05) in phosphatase activity (acid and alkaline phosphatase), protein and phenolics content. The metal toxicities of various metal studied was Co>Ni>Fe. The present study concludes that the Broad Beans ( Vicia faba L.) is a metal resistant plant which have devised various mechanism such as denovo synthesis of anti-stress protein or mitigation of ROS by enhanced phenolic production to combat metal stress.
I. Ahmad, M.S. and Ashraf, M. (2011). Essential roles and hazardous effects of nickel in plants. Rev Environ Contam Toxicol., 214, p.125–167.
II. Asati, A., Pichhodand. and Nikhil., K. (2016). Effect of heavy metals on plants :An overview. 5(3), p.56-66.
III. Ashraf, M.Y., Sadiq, R., Hussain, M., Ashraf, M. and Ahmad MS. (2011). Toxic effect of nickel (Ni) on growth and metabolism in germinating seeds of sunflower (Helianthus annuus L.) Biol Trace Elem Res., 143, p.1695–1703.
IV. De-dorlodot, S., Lutts, S. and Bertin, P. (2005). Effects of ferrous iron toxicity on the growth and mineral composition of an inter specific rice. J. Plant. Nutr. 28, p.1–20.
V. Díaz J., Bernal A., Pomar F., Merino F. (2001). Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification. Plant Science, v. 161, p. 179-188.
VI. Gabbrielli, R., Grossi, L. and Vergnano, O. (1989). The effects of nickel, cadmium and magnesium on the acid phosphatase activity of two Alyssum species. New Phytologist 111, p.631–636.
VII. Hasan, S.A., Hayat, S., Wani, A.S. and Ahmad, A.(2011). Establishment of sensitive and resistant variety of tomato on the basis of photosynthesis and antioxidative enzymes in the presence of cobalt applied as shotgun approach. Braz. J. Plant Physiol. 23(3), p.175- 185.
VIII. Huttova,´ J., Tamas, L. and Mistrik, I. (2002). Aluminium induced acid phosphatase activity in roots of Al-sensitive and Al-tolerant barley varieties. Rostlinna Vyroba 48, p.556-559.
IX. Johnson, R. (2007). Measuring Amylase activity in Cereal Grains. Colby J. Res. Meth., 9, p.11-13.
X. Lowry, O.H., N.J. Rosebrough., A.L. Farr and R.J. Randall, (1951). Protein measurement with the Folin-Phenol reagents. J. Biol. Chem. 193, p.265-275.
XI. Mishra, S., Srivastava, S., Tripathi, R.D., Kumar, R., Seth, C.S. and Gupta, D.K. (2006). Lead detoxification by coontail (Ceratophyllum demersum L.) involves induction of phytochelatins and antioxidant system in response to its accumulation. Chemosphere 65, p.1027-1039.
XII. Mishra, S. and Dubey, R.S. (2008). Changes in phosphate content and phosphatase activities in rice seedlings exposed to arsenite. Braz. J. Plant Physiol., 20, p.19-28.
XIII. Mittal, N., Vaid, P. and Kaur, A. (2015). Effect on amylase activity and growth parameters due to metal toxicity of iron, copper and zinc.,Indian journal of applied research Vol. 5 (4), p.662-664.
XIV. Naguib, D.M. (2016). Enzymatic status of Germinating Wheat grains under Heavy Metal Stress, IJAPSA p. 61-69.
XV. Negi, A., Singh, H. P., Batish, D.R. and Kohli, R. K.( 2014). Ni+2 -inhibited radicle growth in germinating wheat seeds involves alterations in sugar metabolism. Acta. Physiol. Plant, 36, 923–929.
XVI. Pena, L,B., Azpilicueta, C.E. and Gallego, S.M. (2011). Sunflower cotyledons cope with copper stress by inducing catalase subunits less sensitive to oxidation. J. Trace Elem. Med. Biol, 25, p.125–129.
XVII. Rout, J.R., and Sahoo, S.L. (2012) morphologica land protein profile alterations in l. with response to iron stress withania somnifera. Indian J. L. Sci. 2(1), p. 21-25.
XVIII. Singh, G., Agnihotri, R.K., Singh, D.K and Sharma, R. (2013). Effect of lead and nickel on root development and biomass production of black gram (Vigna mungo L.) overcoming through exogenous nitrogen application. Inter. J. Agricul. Sc., 5 (13), p. 1410-1417.
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