TRICHODERMA SPP. AND METAL CHELATOR ENHANCED GROWTH AND YIELDS IN WHEAT PLANTS BY INCREASING ZINC AVAILABILITY

Pankaj Giri, S. N. Pandey

Abstract


A pot experiment of wheat was conducted with five different treatments viz. (I-Control, II-20 mg kg-1 Zn, III-50% compost extract, IV-50% extract+bioagent (Trichoderma spp.) and V-EDTA+Zn 20 mg kg-1) to test the response of wheat plants grown in alluvial soil under warehouse condition. The growth attributes like plant height, fresh and dry matter yield enhanced with addition of compost extract, bioagent and EDTA. Number of tillers plant-1, no. of inflorescence plant-1, inflorescence length plant-1, weight inflorescence-1, no. of grains ear-1, and weight per 100 seeds significantly increased in the plants treated with zinc in combination with compost extract, bioagent and EDTA. The value of total chlorophyll for control plants was 2.80 mg g-1 fresh weight, which were significantly increased by 5.3, 10.7, 14.2 and 23.2% on addition of zinc and its combination with different agents. The activity of catalase in shoot for control plants was 655 mg g-1 fresh weight, which was changed to 685, 705, 755 and 808 for II, III, IV and V level of treatments. The activity of amylase and peroxidase were also found to increase after addition of compost, bioagent and EDTA in leaves of wheat plants. The sugar, protein and proline content were significantly higher in the plants treated with different combinations.


Keywords


Wheat, Reproductive yield, Catalase, Chlorophyll and Trichoderma

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Bassi, R. and Sharma, S. S. 1993. Proline accumulation in wheat seedlings exposed to zinc and copper. Phytochemistry, 33: 1339–1342.

Bates, R. P., Waldren and I. D. Teare. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207.

Bisht, S. S., Nautiyal, B. D. and Sharma, C. P. 2002. Zinc nutrient dependent changes in tomato (Lycopersicon esculantum) metabolism. J. Plant Biol., 29: 159-163.

Chang, Y-C.; Chang, Y-C.; Baker, R.; Kleifeld, O.; Chet, I. 1986. Increased growth of plants in the presence of the biological control agent Trichoderma harzianum. Plant Diseases., 70: 145–148.

Cuevas, V.C. 1991. Rapid composting for intensive rice land use. In Innovation for Rural Development; SEAMEO-SEARCA: Los Baños, Philippines,; Volume 1, pp. 5–10.

Cuevas, V.C., Sinohin, A.M., Orajay, J.I. 2005. Performance of selected Philippine species of Trichoderma as biocontrol agents of damping off pathogens and as growth enhancer of vegetables in farmer’s field. Philipp. Agr. Sci., 88: 63–71.

Dasalkar, J.S., Rudraksha, G.B., law, B.T., Rachewad S.N. 1992. Direct and residual effect of different zinc source on growth, yield and quality of sorghum and wheat. Annals of Plant Physiology, 6: 21-23.

Davidson, J. 1988. Plant beneficial bacteria. Biotechnol., 6: 282–286.

Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. 1956. Calorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350- 356.

Header and Beringer, 1981).

Duxbury, A. C. and Yentsch, C. S. 1956. Plankton pigment nomographs. J. Marine Res., 15: 92-101.

El-Magid, A.A.A.; R. E. Knany and H. G. A. El-Fotoh 2000. Effect of foliar application of some micronutrients on wheat yield and quality. Annals of Agricultural Science Cairo, 1(Special): 301-313.

Euler, H. and Von and Josephson, K. 1927. Liebige Ann. 452:158.

Gamal El-Din, M. Karima. 2005. Physiological response of fenugreek plant to heat hardening and zinc. Egypt. J. Appli. Sci., 20(6B): 400-411.

Goldstein, A.H.; Liu, S.T. 1987. Molecular cloning and regulation of a mineral phosphate solubilizing gene from Erwinia herbicola. Nat. Biotech., 5: 72–74.

Harman, G.E. 2000. Myths and dogmas of biocontrol. Changes in perceptions derived from research on Trichoderma harzianum T-22. Plant Diseases, 84: 377–393.

Harman, G.E. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology, 96: 190–194.

Hassanein, M. S., M. A. F. Shalaby and E. M. Rashad. 2000. Improving growth and yield of some faba bean cultivars by using some plant growth promoters in newly cultivated land. Annals of Agricultural Science, Moshtohor, 38(4): 2141-2155.

Katsuni, M. and Fekuhara, M. 1969. The activity of amylase in shoot and its relation to induced elongation. Physiol. Plan, 22: 68-75.

Lichtenthaler, H. K. and Wellburn, A. R. 1983. Determination of chlorophyll a and b of leaf extract in different solvents. Biochem. Soc. Trans., 11: 591-597.

Lindsey, D.L.; Baker, R. 1967. Effect of certain fungi on dwarf tomatoes grown under gnotobiotic conditions. Phytopathology, 57: 1262–1263.

Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein determination with Folin reagent. J. Biol. Chem., 193: 265-276.

Luck, H. 1963. In Methods of Enzymatic Analysis, 2nd edn. Bergmeyer, H. U., ed.), 885-888, Verlag Chemie, Weinheim, and Academic Press, NewYork.

Majidi, A. and M. J. Malakouti, 1998. Effects of source and the amounts of Zn fertilizers on wheat yield and grain fortification, Soil and Water J., 12(4): 74-83.

Manivasagaperumal, R., Balamurugan, S., Thiyagarajan, G. and Sekar, J. 2011. Effect of Zinc on Germination, Seedling Growth and Biochemical Content of Cluster Bean (Cyamopsis tetragonoloba L.). Curr. Bot., 2: 11-15.

Maralian H, 2009. Effect of foliar application of Zn and Fe on wheat yield and quality. African Journal of Biotechnology, 8: 6795-6798.

Pandey, N., G. C. Pathak, A. K. Singh, and C. P. Sharma. 2002. Enzymic changes in response to zinc nutrition. Journal of Plant Physiology, 159:1151–1153.

Potarzycki, J. and W. Grzebisz. 2009 Effect of zinc foliar application on grain yield of maize and its yielding components. Plant Soil Environ., 55(12): 519-527.

Rashed, M. H. and H. A. Ahmed. 1997. Physiological studied on the effect of iron and zinc supplied on faba bean plant. J. Agric. Sci., Mansoura Univ., 22(3): 729-743.

Romheld, V. and H. Marachner. 1995. Genotypical differences among graminaceous species in release of phytoadiero phores and uptake of iron phytoauid phores. Plant Soil, 132: 147-153.

Saradhi, A. and Saradhi. P.P. 1991. Proline accumulation under heavy metal stress. J. Plant Physiol. 138: 554-558.

Seilsepour M. 2007. The study of fe and zn effects on quantitative and qualitative parameters of winter wheat and determination of critical levels of these elements in Varamin plain soils. Pajouhesh & Sazandegi 76: 123-133.

Shahabifar G, Mostashari M. 2002. Critical point of iron and zinc for wheat production in Qazvin region. Seventh Congress of Agronomy Abstracts. Seed and plant improvement institute. Branch. Iran. P.735.

Shoresh, M.; Harman, G.E. 2008. The molecular basis of shoot responses of maize seedlings to Trichoderma harzianum T22 inoculation of the root: A proteomic approach. Plant Physiol,. 147: 2147–2163.

Singh, D., Kamlesh, N. and Yogesh, K. S. 2007. Response of wheat seed germination and seedling growth under copper stress. J. Environ. Biol., 28: 409-414.

Tobbal, Y. F. M. 1999. Physiological studies on the effect of some micronutrients on growth and metabolism of some plants. M. Sc. Thesis, Fac. Sci. Al-Azhar Univ.

Tobbal, Y. F. M. 2006. Physiological studies on the effect of some nutrients and growth regulators on plant growth and metabolism. Ph.D. Thesis, Fac. Sci. Al-Azhar Univ.

Venekamp, J. H. 1989. Regulation of cytosol acidity in plants under conditions of drought. Physiol. Plant. 76: 112–117.

Weindling, R. 1932. Trichoderma lignorum as a parasite of other soil fungi. Phytopathology, 22: 837–845.

Wu, J. T., S. C. Chang and K. S. Chen. 1995. Enhancement of intracellular proline level in cellof anacystis nidulans (Yanobacteria) exposed to deleterious concentrations of copper. J. physiol. 31 in press).

Yedidia, I.; Srivastva, A.K.; Kapulnik, Y.; Chet, I. 2001 Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant Soil, 235: 235–242.

Yilmaz A, Ekiz H, Torun B, Gultekin I, Karanlik S, Bagci SA, Cakmak I 1997. Effect of different zinc application methods on grain yield and zinc concentration in wheat grown on zinc-deficient calcareous soils in Central Anatolia. J. Plant Nutr., 20: 461-471.


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